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h 1400 10 superfrost plus adhesion microscope slides epredia  (Vector Laboratories)


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    Vector Laboratories h 1400 10 superfrost plus adhesion microscope slides epredia
    H 1400 10 Superfrost Plus Adhesion Microscope Slides Epredia, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+1400+10+superfrost+plus+adhesion+microscope+slides+epredia/VECTASHIELD+Hardset+Antifade+Mounting+Medium/pmc12147903__mmc2-242-279-277
    Average 96 stars, based on 2533 article reviews
    h 1400 10 superfrost plus adhesion microscope slides epredia - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 Article ll OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Sterility:

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 Article ll OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    SYBR Green Assay:

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 Article ll OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Microscopy:

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 Article ll OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s

    Article Title: RSPO1, a potent inducer of pancreatic β cell neogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLEX Polyclonal Guinea Pig Anti-Insulin Dako Cat# IR00261-2 Rat Monoclonal anti-BrdU antibody Abcam Cat# ab6326, RRID:AB_305426 Anti-KI67 antibody, Rabbit monoclonal Sigma Aldrich SAB5600050 Goat anti-Guinea Pig IgG Alexa Flour-488 Thermo Fisher Cat# A-11073, RRID:AB_2534117 Goat anti-Guinea Pig IgG Alexa Flour-594 Thermo Fisher Cat# A-11076, RRID:AB_2534120 Donkey anti-Rat IgG Alexa Fluor 488 Thermo Fisher Cat# A-21208, RRID:AB_141709 Donkey anti-Rat IgG Alexa Fluor 594 Thermo Fisher Cat# A-21209, RRID:AB_2535795 Donkey anti-Rabbit IgG Alexa Fluor 488 Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-Rabbit IgG Alexa Fluor 594 Thermo Fisher Cat# A-21207, RRID:AB_141637 Chemicals, peptides, and recombinant proteins DPBS Fisher Scientific Cat# 12559069 0,25% sterile-filtered trypsin-EDTA solution Fisher Scientific Cat# 25200-056 Heat-inactivated Fetal Calf Serum (FCS) Sigma Aldrich Cat# ES-009-B Penicillin Streptomycin Gibco Cat# 15140-122 50μM β-mercaptoethanol Thermo Fisher Cat# 31350 DMEM high-glucose Gibco Cat# 10569010 MSAB Merck Cat# SML1726-5MG DMSO Merck Cat# 20-139 Cardamonin Merck Cat# C8249 Bovin Serum Albumin BSA Sigma Aldrich Cat# A19418 DMEM low-glucose Gibco Cat# 31885-023 Collagenase Sigma Aldrich Cat# C7657 1119 Histopaque Sigma Aldrich Cat# 1119 1077 Histopaque Sigma Aldrich Cat# 1077 HBSS Gibco Cat# 14175-053 RPMI-1640 Sigma Aldrich Cat# R8758 BrdU (5-bromo-2′-désoxyuridine) Sigma Aldrich Cat# 19-160 D-(+)-glucose Sigma Aldrich Cat# G8270 K3EDTA collection tubes Starstedt Cat# 41.3395.005 RNAlater solution Invitrogen Cat# AM7024 RNeasy Mini Kit Qiagen Cat# 74104 10nM dNTP mix Invitrogen Cat# 18427089 50nM Oligo(dT) primer Invitrogen Cat# AM5730G MgCl2 (chlorure de magnésium) (25 mM) Thermo Fisher Cat# R0971 M-MLV RT Buffer Invitrogen Cat# 18057018 RNaseOUT Invitrogen Cat# 10777019 Superscript III Invitrogen Cat# 18080093 RNase H Invitrogen Cat# 18021014 LightCycler® 480 SYBR Green I Master Roche Cat# 04887352001 Paraplast Sigma Aldrich Cat# P3558 VECTASHIELD HardSet Mounting Medium Vector Cat# H-1400-10 Superfrost Plus Adhesion Microscope Slides Epredia Cat# J1800AMNZ (Continued on next page) Cell Reports Medicine 6, 102126, May 20, 2025 e1 OPEN ACCESS .. The pancreatic immortalized β-cell lines MIN6 (AddexBio) and Ins1 (ATCC), the α-cell-derived αTC1 (ATCC) and the acinar cell line 266-6 (ATCC) were cultured in 100mm Petri dishes (Eppendorf) in cell line-specific culture media indicated by manufacturer’s



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    Vector Laboratories h 1400 10 superfrost plus adhesion microscope slides epredia
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